LOCUS       40924_4115       10891 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pAgc-A3-bgyg-3'N DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Ikeda Y, Ito K, Izumi Y, Shinomura T.
  TITLE     A candidate enhancer element responsible for high-level expression 
            of the aggrecan gene in chondrocytes
  JOURNAL   J. Biochem. 156 (1), 21-28 (2014)
  PUBMED    24554731
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   Ikeda Y, Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and 
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/
REFERENCE   3  (bases 1 to 10891)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2014"; volume: "156"; issue: "1"; pages: "21-28"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan URL :http://www.tmd.ac.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      18..546
                     /gene="bgyg"
                     /label=rat Agc1 promoter sequence
                     /note="rat Agc1 promoter sequence"
                     /regulatory_class="promoter"
     misc_binding    1511..1786
                     /gene="bgyg"
                     /label=SOX9 binding site
                     /bound_moiety="SOX9"
                     /note="A3"
     CDS             2678..5719
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             5735..6769
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     promoter        complement(7760..7778)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7796..7812)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7820..7836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7844..7874)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7889..7910)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8198..8786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8960..9817)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9818..9922)
                     /label=AmpR promoter
     rep_origin      10256..10711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     10852..10868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcgaattc gagctcgccc acccccaaaa ctttccaaca ctgttctgta agggttcttt
       61 caagtagcat tcccagggta gggggtcgtc ctgcgcgatc gcatctttct gggtctcagc
      121 tgcaaatcgc ctccaggtat ggggggggaa ccacccctcc ccgcggccag ggcctgtcat
      181 tctgacagca ggagtcgggt gctttcggca ctctgcgggc acagccctcc ggggcctcct
      241 ggactgcgga gcttacgacc gcaatgcaga cccgggtcct ccagcgctac cagcacagct
      301 ttcctccgcg gccccaggag ctgcggggtc cgcgctcctc tcgtgccctg cgcgcccggc
      361 gccttcccca gtgagcgcgg gtccccagcc ctggtccaga gcgctccgga cgtctctgcc
      421 ttcccctccc cctgcgaact ggccccgggg gtggggtttc cctgtgcgct cgcccccacc
      481 cctagtttgt gccctcccct cccccgcccg cccctatgta tgtgtcaccg cgcaccattc
      541 ccgcccacct acctccccgc ggtgccagag gggctcacag agctgaggac gcgtgcagta
      601 ccgcccaagg tccctcattc tccgcgacta gcatctgaca catgtgccga aggaactcaa
      661 gacacctttc tgtatccctt ccctccatcg accctgctcc gaaacccagc cagccagcta
      721 ccactgcggc caccgcccga ggggacttgc ggaggagacg ccggcgggag gagggcttcg
      781 cagcccccac ccccaccccc actcccagcc cagagcatcc ctgctgcagc gcagcaagac
      841 ccggggctgc cagcccccag gaatccctag ctgcttagca gggataacgg actgaagttc
      901 ttggaggagc gagttcaact cttcaaggta aagaaagggc tccttctctc cagaggagct
      961 ctgtcccagg gcatctggga atgctgctga ggacgcgcgc ctaagtgctg ggggtggcgt
     1021 gccttggtgc ccacattgag tatgtgtgtt ggggactcag gacattagct gcatgcccat
     1081 ggaagttgag caaactcaac tgctgccccg actcaaagtg aatgggaaca cgtggagggg
     1141 ctcctgctca gagtacacag aattaagaaa gaccagagct ttttcaagtt taaagtaccg
     1201 gggcgctaaa ctgaagtgta gagcgagaaa cagttttaaa gccaaggaaa tgtgtgttca
     1261 aaccccactc ccccccgcaa cccctcgttc ctctgggcgc tccataaggc tttcatattc
     1321 tcttgggaag gccaactgat cagagaatca gcaaaattat gtcgaggggg ggcccggtac
     1381 ctctggaaca accagatgcc acctggcctg gcctccacca gacctcttcc tttcctgccc
     1441 tttgtgtcaa ggttgggcca agggctccag cgttggtgtc tgacttttct tccctctgag
     1501 gtcaccccat ccctgctcta tacaactgct ttagcgcctc catttgctta accccttacc
     1561 ctagtgcttc tggcctccca taatgaagct aatggacaaa agagaacatg attatctttc
     1621 ctgaagctgg agagccgccc ccagcttcca ctttgccaac tgacctgacc tattgctgct
     1681 actgctgctg ctgggaatcc tggtgcatgc tgggaaacca agtgcatgcc gggatttcag
     1741 tcagttcctc catgcatttt tggggttgct tggaactgtg agtctctaat ccgtggcgcg
     1801 tgcttcttga aatgaagcta agaacttcgt gagaagagaa gagaggccag cgacatggag
     1861 aagaagggca gcaccggttg aggaaggtac ccggggatcc cccgggctgc agagactccc
     1921 aggaggccaa ggcagctggc tgcacatact cagtttgaag ctccacatgt tcaagcccca
     1981 ggaaaagctg atgcctcagg tcaagtccaa agggaggaga aagtcagcgg gaaggccatc
     2041 agggaagaag tctctctccc tccacagagg gtcctttttt gtttaaagcc ttcatggggg
     2101 tggctgtgac ccacccacac tgaaaaggga ggccattcta ggtctacaga ctccagagtt
     2161 tatgcaaaac ccctcacaga tatagaagag agcataaacc tgagcatcta tggtccgcct
     2221 gtcacatatc actggctacc ccaggaactc attaggatga gtctccactg gctaggagat
     2281 gggggaacca gtacaccagc catcttggaa ttaagctaag aaaggtcgaa ggtttctgag
     2341 gaaaggattc ttagtgaaaa cactgtccaa accatgacaa cccgctgttg tgcaatgtga
     2401 cttcagagcc ttgggcatga attttatcac ttttttttct tcctttaact atcccgtgct
     2461 ttaactgtcc aaataacatc cctgttgtct cttccagctg aactctgtcg acggtatcga
     2521 taagcttgga tccccagctt gggggatcga aagagcctgc taaagcaaaa aagaagtcac
     2581 catgtcgttt actttgacca acaagaacgt gattttcgtt gccggtctgg gaggcattgg
     2641 tctggacacc agcaaggagc tgctcaagcg cgatcccgtc gttttacaac gtcgtgactg
     2701 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2761 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2821 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
     2881 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
     2941 tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac
     3001 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
     3061 aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
     3121 gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt
     3181 tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg acggcagtta
     3241 tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca
     3301 taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag
     3361 ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
     3421 aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg
     3481 tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga
     3541 aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca
     3601 caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg
     3661 gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg
     3721 tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat
     3781 cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
     3841 gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga
     3901 aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat
     3961 gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg
     4021 gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
     4081 atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
     4141 cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
     4201 gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
     4261 cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt
     4321 tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat
     4381 taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc
     4441 gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc
     4501 gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat
     4561 cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt
     4621 ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
     4681 taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct
     4741 cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc
     4801 ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
     4861 catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
     4921 gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
     4981 gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
     5041 tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
     5101 ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat
     5161 tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta
     5221 ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac
     5281 accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa
     5341 ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga
     5401 ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg
     5461 tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
     5521 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
     5581 gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
     5641 cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta
     5701 ccagttggtc tggtgtcagg ggatcctcgc caccatgaaa aagcctgaac tcaccgcgac
     5761 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     5821 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     5881 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     5941 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     6001 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     6061 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     6121 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     6181 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     6241 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     6301 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     6361 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     6421 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     6481 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     6541 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     6601 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     6661 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     6721 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gacccgggac
     6781 tagtcgacct gcagccaaca cctacaagca caggctacag aagcggagca tgagacccac
     6841 acggaggagc cgccccagca tggcccactg agaggagctt ccacaatgtg cccaggatgc
     6901 tgagcccagc ggccagccat gctgaccgtg catcccactc acatggtgtc ttcttgtcgc
     6961 tttttgtcat ataaggaatc cattaaagaa ggaaaaaaaa gccacattgt gtgtgcccca
     7021 ctccccatat cacccaaact gcatctaatt tatccaccac catcaccgcc ccccataaaa
     7081 aaccgccaaa gcaaattcac tgtaacccat ggactgagtt tagagacatt tctttcattt
     7141 cccaccgtgc ctttccaggg accagtgcag ggacagggtg agaagagtta aattaaataa
     7201 agaagattat tttttgttgt tgcttcctga ctttacccaa gaacagcaca attgttggtt
     7261 acttcacctc cagggagagc taggggccag aggtggaatt ggaaggagac agggccaggg
     7321 agagaggagt agggcagcca cacagaccag agctttaagg actcagaaga ggcaaactat
     7381 agcttagtgt gttggacaaa agggatcgag gtctgtgcca tctatgtggg aaggagcctg
     7441 gggcaggggg caggctggcc aaagggaagt gaagttgcct ttcctccagg gcccctctcc
     7501 taggaacctt tctcctagga acctttcttc tgggtcaacc accctagcag gtgtcatttc
     7561 tcggctggac tgggtagggg cacttccttc ccaggagtgc tccgccgtcc ccttccctct
     7621 ccccaccccg ggacggtgca ggcaccagtg ttccgtgcac ctatttatat ttttgaaaac
     7681 taaagattat gataattata ataataaaga cattggaaga gatctgctag ctcgagaatt
     7741 cgatatcaag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
     7801 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7861 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7921 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7981 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8041 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8101 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8161 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt cgataggctc cgcccccctg
     8221 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8281 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8341 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8401 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8461 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8521 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8581 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8641 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8701 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8761 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8821 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8881 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8941 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9001 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9061 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9121 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9181 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9241 ttaatagttt gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca cgctcgtcgt
     9301 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9361 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9421 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9481 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaataccgcg
     9541 cccggcgacc gagttgctct tgcccggcgt caatacggga taatagtgta tgacatagca
     9601 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9661 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9721 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9781 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9841 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9901 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9961 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10021 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10081 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10141 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10201 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgacgcg
    10261 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10321 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10381 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagagct
    10441 ttacggcacc tcgaccgcaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
    10501 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10561 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10621 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaat atttaacgcg
    10681 aattttaaca aaatattaac gtttacaatt tccattcgcc attcaggctg cgcaactgtt
    10741 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    10801 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10861 cggccagtga attgtaatac gactcactat a
//
